FIG. 1.

(A) Schematic linear diagrams of the transformation plasmids. Relevant restriction sites are marked below the diagram. The portion of the plasmid used as a probe in the Southern blots are indicated with hatched boxes. (B, C, D, and E) Southern blot analysis of the transformants. H99 is the parental strain. H1 to H16 are stable hygromycin-resistant H99 transformants, G1 to G16 are G418-resistant H99 transformants, and P1 to P16 are phleomycin-resistant H99 transformants. Some of the G418 and phleomycin resistant transformants were stable, and those are marked above the lane. The size markers are indicated along the left side of the blots. Whether the genomic DNA is uncut or restricted with EcoRI is indicated below each blot. Uncut genomic DNA migrates with the 23.1-kb size marker. The position at which the linearized parent plasmid migrates is marked on the right of each blot with an arrow.