Figure 1. Cancer secreted OPN induces MSC adoption of myCAF phenotype resulting in increased cancer stemness.

Co-culture of wild type MDA-MB-231 cells with human MSC cells and cancer stemness lacking MDA-MB-231(-/-sox2) cells with human MSC cells. Cancer stemness inhibitor BBI-608 was added as indicated: (A) cancer stemness marker Sox2 mRNA expression was quantified by real time PCR and (B) myCAF marker α-SMA mRNA expression was quantified by real time PCR.