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Co-culture of wild type MDA-MB-231 cells with human MSC cells and cancer stemness lacking MDA-MB-231(-/-sox2) cells with human MSC cells. Cancer stemness inhibitor BBI-608 was added as indicated (A,B) Cancer stemness markers Oct4/Nanog mRNA expression were quantified by real time PCR. (C,D) myCAF markers Vim/Ten-C mRNA expression were quantified by real time PCR.
