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. 2022 Oct 14;136(19):1405–1423. doi: 10.1042/CS20210889

Figure 5. Inhibition of β-catenin-dependent Wnt signalling by ICG-001 blocks both steady state and TGFβ-induced Collagen-I up-regulation.

Figure 5

(A) Levels of β-catenin, Collagen-I, and cell numbers were evaluated by IF (n=4). (B) Representative western blots for β-catenin, FZD8, and Fibronectin; these data are normalised to the loading control β-Actin (n=4). (C–I) Protein quantifications from IF and western blots. (D) Pro-Collagen-Iα1 levels in the cell media, measured by ELISA, are also provided (n=4). (J) The ability of ICG-001 to suppress markers of TGFβ-induced myofibroblast activation in CCD-18Co cells was also assessed by qPCR and the data presented in a bar graph (n=4). (K) ICG-001 effects on gel remodelling and pro-Collagen-Iα1 were also confirmed in a 3D organotypic model in the absence of TGFβ (n=4); DL = density levels. Differences between treatments were determined by a paired t-test to account for different cell passages. In general, data are presented as fold-changes with panels C–I and K(i–ii) as box plots showing 25th to 75th percentiles, median (horizontal bar), and the smallest and largest value (whiskers). Panel J shows mean ± SEM. Significant results relative to control are indicated by * symbol (*<0.05, **<0.01, ***<0.001). A bar indicates specific statistical comparisons.