Figure 1.
HIF-1α ablation in rods significantly reduced HIF-1α expression in the retina and leaded to compensatory increase of HIF-2α protein. (A) Relative Hif1a mRNA levels in naïve attached and detached retinas of Rho-Cre+ and HIF-1αΔrod mice at 4 hours and 1 day post retinal detachment (RD). Hif1a mRNA levels were normalized to the ribosomal protein L19 mRNA levels (Rpl19; N = 4). (B) Western blot analysis of HIF-1α and HIF-2α protein levels in naïve attached and detached retinas of HIF-1αΔrod and control mice at 3 days post RD. Rho-Cre+ mice were used as controls. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) served as loading controls. Quantification of protein levels based on densitometry of the Western blots are shown in graphs (N = 3). Data are shown as means ± SD. One-way ANOVA and Tukey's test for multiple comparisons were used for statistical analysis. * P < 0.05, **P < 0.01, ***P < 0.001.