Figure 2.
Rod-specific HIF-1α ablation resulted in more apoptosis of PR cells after RD. (A) Terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) assay was used to detect apoptotic cells in the outer nuclear layer of HIF-1αΔrod and control mice at 3 days post RD. Rho-Cre+ mice were used as controls. Nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI). Green: TUNEL positive cells, blue: DAPI. Scale bar = 100 µm. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium. (B) Quantification of TUNEL positive cells in the ONL in the attached and detached retinas from the optic nerve head to the extreme retinal periphery. Four biological replicates were obtained in each group. The number of TUNEL positive cells for each replicate was the average number from three noncontinuous retinal sections. Data are shown as mean ± S.D. * P < 0.05 (unpaired Student's t-test).