Figure 2.
Cell type-specific expression of eicosanoid synthases and receptors in murine PanIN. (A) UMAP of a murine PanIN scRNA-seq data set, generated from the study by Schlesinger et al,40 annotated by cell type. (B) Dotplot of average and percent expression of select eicosanoid synthases and receptors in each cell type identified. (C) UMAPs depicting gene expression of select eicosanoid synthases. (D) Co-IF for COX1 (yellow), ALOX5 (magenta), and acetylated α-tubulin (white) in a KC pancreas. DAPI, blue. Scale bar, 10 μm. (E) UMAPs depicting gene expression of select eicosanoid receptors. Color intensity in (C) and (E) indicates the normalized gene expression level for a given gene in each cell. (F) Table summarizing inferred cellular sources of eicosanoids based on synthase expression patterns.