Fig. 2.

Lactate administration decreased VE-cadherin junctions and tight junctions after CLP. A, Mice were subjected to CLP or sham surgery followed by PBS or lactic acid (pH 6.8, 0.5 g/kg body weight) i.p. injection. Twenty-four hours later, heart tissues were collected and protein was isolated. The levels of VE-cadherin and claudin 5 in the myocardium were examined by Western blot. n = 4/group. B, Human umbilical vein endothelial cells were treated with l-lactic acid (10 mM) for 6 h, and protein of HUVECs was then isolated. The expression of VE-cadherin, claudin 5, and ZO-1 was examined by Western blot. n = 3/group. *P < 0.05, **P < 0.01, ***P < 0.001 compared with indicated group.