Table 3.
Primers used in this study for qRT-PCR analysis
| Gene name1 | Accession number | Primer and probe sequences (5ʹ to 3ʹ)2 |
|---|---|---|
| GAPDH1 | NM_001206359.1 | F: TCGGAGTGAACGGATTTGGC R: TGACAAGCTTCCCGTTCTCC |
| GAPDH2 | NM_001206359.1 | F: CGGAGTGAACGGATTTGGC R: CACCCCATTTGATGTTGGCG |
| SOD-1 | NM_001190422.1 | F: AAGGCCGTGTGTGTGCTGAA R: AGTGGCCACACCATCTTTGC |
| SOD-2 | NM_214127.2 | F: GGCCTACGTGAACAACCTGA R: TGATTGATGTGGCCTCCACC |
| SOD-3 | NM_001078688.1 | F: TGACGCTGCTCTGTGCTTAC |
| R: AACTCCTGCCAGATCTCCGT | ||
| CAT | NM_214301.2 | F: CCTGCAACGTTCTGTAAGGC |
| R: GCTTCATCTGGTCACTGGCT | ||
| GPX-1 | NM_214201.1 | F: CCTAGCAGTGCCTAGAGTGC |
| R: CGCCCATCTCAGGGGATTTT | ||
| GPX-2 | NM_001115136.1 | F: CTGGACGGGGAGAAGGTAGA |
| R: CGGACGTACTTGAGGCTGTT | ||
| ZO-1 | XM_021098856.1 | F: TCAAGGTCTGCCGAGACAAC |
| R: ATCACAGTGTGGTAAGCGCA | ||
| Claudin-2 | NM_001161638.1 | F: AACGAGTTCTTACGTCGGGG |
| R: CGAGGAGATGGCGCTAGATG | ||
| Claudin-5 | NM_001161636.1 | F: CCTGTCAAGTATTCGGCCCC |
| R: CGACACCCTCAGACGTAGTT | ||
| Occludin | NM_001163647.2 | F: CAGGTGCACCCTCCAGATTG |
| R: ATGTCGTTGCTGGGTGCATA |
GAPDH, Housekeeping gene; SOD, superoxide dismutase; CAT, catalase; GPX, glutathione peroxidase; ZO-1, zonula occludens-1.
F, forward; R, reverse.