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. 2022 Oct 19;6(11):e795. doi: 10.1097/HS9.0000000000000795

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Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 1. — Morphological features, conventional and molecular cytogenetics, and expression of IL3 in the index case with t(5;7)(q31;q21)/CDK6::IL3. (A and B) Leukemic blasts and eosinophils in the peripheral blood (A) and bone marrow (B) (May-Grunwald Giemsa stain) (Microscope Zeiis, magnification 100×; Camera AXIOCAM 105 color; Software ZEN Imaging Software 2.3); (C) Representative G-banded karyotype of the index case. Arrows indicate chromosomes 5 and 7 involved in reciprocal t(5;7)(q31;q21) (Microscope Olympus BX61, magnification 100×; Camera GENASIS Scanning System, ASI; Software GENASIS Band View, ASI); (D) Double color double fusion FISH experiment on an abnormal metaphase shows two fusion signals on der(5) and der(7), 1 orange signal, on normal chromosome 5, and 1 green signal on normal chromosome 7 (Microscope Olympus BX61, magnification 100×; Camera JAI progressive scan; Software CytoVision [Leica Microsystem]); (E) Schematic representation of DNA clones used for the CDK6::IL3 dual-color fusion assay, and their position relative to CDK6 and IL3. The red arrowhead indicates the position of the CDK6 enhancer; (F) A graph showing the expression levels of IL3 in our cohort of 159 T-ALL cases (RNA microarray data). The red dot corresponds to the index case with the CDK6::IL3 rearrangement; (G) Index case: longitudinal study of IL3 expression levels on samples taken at diagnosis and at different time points during therapy and after HSCT (see also Suppl. Table S1); (H) Validation of RNA microarray data by quantitative RT-PCR: IL3 expression in the index case, at diagnosis (CDK6::IL3) and during treatment (time points TP2 and pre-HSCT), and in 11 ETP-ALL cases without CDK6::IL3 (wt). ASI = applied spectral imaging; ETP-ALL = early T-cell precursor acute lymphoblastic leukemia; HSCT = hematopoietic stem cell transplantation; T-ALL = T-cell acute lymphoblastic leukemia.