FIG 2.

Physiological impact of gene disruption for those preferentially expressed in biofilms. Crystal violet biofilm assays were performed with transposon mutants of select genes preferentially expressed within biofilms. Alterations in biofilm mass are reported as a percentage of the wild type. Assays were performed in biological triplicate with 3 technical replicates each. Error bars represent the ± standard error of the mean SEM; Student’s t test was used to determine statistical significance. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.