FIG 7.

CspC mutation results in extended mRNA half-life for transcriptional regulators adeL and ABUW_RS06565. (A and B) Exponentially growing wild-type and cspC mutant strains were treated with 250 μg/mL rifampin to arrest transcription, and changes in transcript abundance were measured by RT-qPCR for adeL (A) and ABUW_RS06565 (B). Values represent the mean fold change in transcript abundance relative to that immediately prior to rifampin treatment (t = 0). Data are from triplicate cultures ± SD. The lines shown are the exponential, one-phase decay curve, represented as R(t) = R₀e^−kt, which were used to calculate mRNA half lives.