qPCR analysis of plaque-isolated DNA from seven root caries patients for Prevotella denticola (A), Propionibacterium acidifaciens (B), Prevotella multisaccharivorax (C), Delftia acidovorans (D), and Capnocytophaga granulosa (E) (n = 6). All data are presented as aggregates of carious or healthy tooth colonization levels per patient. Tooth pair analysis is shown in Fig. S5 in the supplemental material. *, P ≤ 0.05 by the Mann-Whitney test for multiple teeth and Student’s t test if only one carious and one healthy tooth was present in the patient (patients 1 and 4).