TABLE 2.
Cytolytic granule/proliferation flow cytometry panel used for PBMCa
| Antibody | Clone | Tetramer/surface/intracellular |
|---|---|---|
| Gag181-189CM9-PE | — | Tetramerb |
| Tat28-35SL8-BV605 | — | Tetramerb |
| MR1 5-OP-RU-BV421 | — | Tetramer |
| CD3-AF700 | SP34-2 | Surface |
| CD4-BUV737 | SK3 | Surface |
| CD8-BUV395 | RPA-T8 | Surface |
| CD28-BV510 | CD28.2 | Surface |
| CD95-PE Cy7 | DX2 | Surface |
| CCR7-BV711 | G043H7 | Surface |
| LIVE/DEAD Near IR ARD | — | — |
| ki-67-AF488 | B56 | Intracellular |
| GranzymeB-PE CF594 | GB11 | Intracellular |
| Perforin-AF647 | PF344 | Intracellular |
a
—, not applicable.
b
The Gag181-189CM9-PE and Tat28-35SL8-BV605 tetramers were used to characterize SIV-specific conventional T cells, which are described elsewhere (39).