FIG 5.
MroQ is not required for Agr type III activation. (A) TCA-precipitated exoproteins and (B) Hla and HlgC immunoblots from MW2, ΔmroQ, ΔmroQ + mroQ, Δagr::tet, and Δagr::tet ΔmroQ strains. (C) Rabbit red blood cell lysis of cell-free culture filtrates derived from MW2, ΔmroQ, ΔmroQ + mroQ, Δagr::tet, and Δagr::tet ΔmroQ strains. (D) pDB59 reporter activity (RFU/OD600) of LAC (left) and SA502A (right) upon addition of conditioned medium from MW2, ΔmroQ, ΔmroQ + mroQ, Δagr::tet, and Δagr::tet ΔmroQ. (E) TCA-precipitated exoproteins from RN3984, ΔmroQ, and Δagr::tet strains. (F) Hla and HlgC immunoblots of from RN3984, ΔmroQ, and Δagr::tet strains. (G) Rabbit red blood cell lysis of cell-free culture filtrates derived from RN3984, ΔmroQ, and Δagr::tet strains. Hemolysis and reporter assay data are from one of at least three experiments conducted in triplicate. Immunoblots and GelCode blue-stained gels are representative of at least four replicates. Means ± SD are shown (n = 3). ****, P < 0.0001 by one-way ANOVA with Tukey’s posttest.