TABLE 1.
Strains used in this study
| Strain | Description | Designation | Reference or source |
|---|---|---|---|
| AH-1263 | S. aureus USA300 CA-MRSA strain LAC, type I Agr | LAC | 58 |
| SA502A | S. aureus clinical isolate SA502A, type II Agr | SA502A | 59 |
| MW2 | S. aureus CA-MRSA strain, type III Agr | MW2 | 61 |
| RN3984 | S. aureus clinical isolate, type III Agr | RN3984 | 61 |
| RN4850 | S. aureus clinical isolate, type IV Agr | RN4850 | 62 |
| BH10C | E. coli strain that restricts plasmid copy no. for cloning of mroQ | BH10C | 66 |
| DH5α | E. coli strain used for cloning | DH5α | |
| RN4220 | Restriction-negative S. aureus | RN4220 | 67 |
| RN9011 | RN4220 with pRN7023 expressing the SaPI-I integrase | RN9011 | 68 |
| FA-S922 | LAC with in-frame deletion of mroQ | ΔmroQ | 21 |
| FA-S982 | FA-S922 with integrated pJC1112-mroQ for complementation | ΔmroQ + mroQ | 21 |
| FA-S1008 | LAC with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet | 21 |
| FA-S995 | FA-S922 with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet ΔmroQ | 21 |
| FA-S2733 | SA502A with in-frame deletion of mroQ | ΔmroQ | This work |
| FA-S2764 | FA-S2733 with pOS1-FLAG-GG-mroQ for complementation | ΔmroQ + mroQ | This work |
| FA-S2766 | SA502A with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet | This work |
| FA-S2741 | FA-S2733 with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet ΔmroQ | This work |
| FA-S2441 | MW2 with in-frame deletion of mroQ | ΔmroQ | This work |
| FA-S2454 | FA-S2441 with integrated pJC1112-mroQ for complementation | ΔmroQ + mroQ | This work |
| FA-S2735 | MW2 with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet | This work |
| FA-S2738 | FA-S2441 with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet ΔmroQ | This work |
| FA-S2730 | RN3984 with in-frame deletion of mroQ | ΔmroQ | This work |
| FA-S2734 | RN3984 with gene replacement of agrBDCA with tetracycline resistance cassette | Δagr::tet | This work |
| FA-S949 | LAC with P3-GFP reporter plasmid pDB59 | LAC + pDB59 | 69 |
| FA-S1972 | AH-1263 with an in-frame deletion of agrB and P3-GFP reporter plasmid pDB59 | ΔagrB + pDB59 | 21 |
| FA-S1881 | SA502A with P3-GFP reporter plasmid pDB59 | SA502A + pDB59 | 69 |
| FA-S2347 | MW2 with P3-GFP reporter plasmid pDB59 | MW2 + pDB59 | 69 |
| FA-S2767 | RN4850 with P3-GFP reporter plasmid pDB59 | RN4850 + pDB59 | 69 |
| FA-S2005 | AH-1263 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | LAC pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2010 | FA-S922 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | ΔmroQ pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2008 | FA-S982 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | ΔmroQ + mroQ pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2422 | SA502A containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-II | SA502A pOS1-PsarA-sodRBS-6×-His-GG-agrD-II | This work |
| FA-S2746 | FA-S2733 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-II | ΔmroQ pOS1-PsarA-sodRBS-6×-His-GG-agrD-II | This work |
| FA-S2383 | MW2 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | MW2 pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | This work |
| FA-S2452 | FA-S2441 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | ΔmroQ pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | This work |
| FA-S2762 | FA-S2454 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | ΔmroQ + mroQ pOS1-PsarA-sodRBS-6×-His-GG-agrD-III | This work |
| FA-S2028 | FA-S1008 with integrated pJC1111-agr-I | Δagr::tet + agr-I | This work |
| FA-S2030 | FA-S995 with integrated pJC1111-agr-I | Δagr::tet ΔmroQ + agr-I | This work |
| FA-S2032 | FA-S1008 with integrated pJC1111-agr-II | Δagr::tet + agr-II | This work |
| FA-S2034 | FA-S995 with integrated pJC1111-agr-II | Δagr::tet ΔmroQ + agr-II | This work |
| FA-S2036 | FA-S1008 with integrated pJC1111-agr-III | Δagr::tet + agr-III | This work |
| FA-S2038 | FA-S995 with integrated pJC1111-agr-III | Δagr::tet ΔmroQ + agr-III | This work |
| FA-S2040 | FA-S1008 with integrated pJC1111-agr-IV | Δagr::tet + agr-IV | This work |
| FA-S2042 | FA-S995 with integrated pJC1111-agr-IV | Δagr::tet ΔmroQ + agr-IV | This work |
| FA-S2728 | FA-S2735 with integrated pJC1111-agr-I | MW2 Δagr::tet + agr-I | This work |
| FA-S2750 | FA-S2738 with integrated pJC1111-agr-I | MW2 Δagr::tet ΔmroQ + agr-I | This work |
| FA-S2530 | LAC with an in-frame deletion of agrD | ΔagrD | This work |
| FA-S2564 | FA-S922 with an in-frame deletion of agrD | ΔmroQ ΔagrD | This work |
| FA-S2718 | FA-S982 with an in-frame deletion of agrD | ΔmroQ ΔagrD + mroQ | This work |
| FA-S2526 | FA-S2530 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | ΔagrD pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2577 | FA-S2564 containing pOS1-PsarA-sodRBS-6×-His-GG-agrD-I | ΔmroQ ΔagrD pOSI-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2772 | FA-S2718 containing pOSI-PsarA-sodRBS-6×-His-GG-agrD-I | ΔmroQ ΔagrD + mroQ pOSI-PsarA-sodRBS-6×-His-GG-agrD-I | This work |
| FA-S2525 | FA-S2530 containing pOS1-PsarA-sodRBS-6×-His-GG-Leader-I | ΔagrD pOS1-PsarA-sodRBS-6×-His-GG-Leader-I | This work |
| FA-S2583 | FA-S2564 containing pOS1-PsarA-sodRBS-6×-His-GG-Leader-AIP-I | ΔmroQ ΔagrD pOS1-PsarA-sodRBS-6×-His-GG-Leader-AIP-I | This work |