TABLE 2.
Oligonucleotides used in this study
| Name | Sequence |
|---|---|
| MroQ-1 | CCC-GGTACC(KpnI)-CCATAAATGATAAACCTCCAT |
| MroQ-2 | GTGTGATTCGTTTTTTTTATTA-GGCGCC(KasI)-CATAATTTTCCTCCAAATATT |
| MroQ-3 | AATATTTGGAGGAAAATTATG-GGCGCC(KasI)-TAATAAAAAAAACGAATCACAC |
| MroQ-4 | CCC-GAGCTC(SacI)-ATTTTTAGCCTTGGCAAATG |
| MroQFwd | ATGACAAGATTATGGGCATCAT |
| MroQRev | TTATGGAATAAAAATGTGATAT |
| pOS1UniSOE1 | CCC-CTGCAG(PstI)-CTGATATTTTTGACTAAACCAA |
| PsarA-sodRBS-6×-His-GG-agrD-SOE2 | ACCACCGTGATGGTGATGGTGATGGCTGCTGCCCAT-AAATAATCATCCTCCTAAGGT |
| PsarA-sodRBS-6×-His-GG-agrDI-SOE3 | ATGCATCACCATCACCATC-CCTTAGGAGGATGATTATTT |
| PsarA-sodRBS-6×-His-GG-agrDI-SOE4 | ATAT-GAATTC(EcoRI)-TTATTCGTGTAATTGTGTTAAT |
| PsarA-sodRBS-6×-His-GG-agrDII-SOE3 | ATGGGCAGCAGCCATCACCATCACCATCACGGTGGT-AATACACTTGTTAATATGTTTTTT |
| PsarA-sodRBS-6×-His-GG-agrDII-SOE4 | CCC-GAATTC(EcoRI)-CTATTTGTCGTATAAATTCGTT |
| PsarA-sodRBS-6×-His-GG-agrDIII-SOE3 | CATCACCATCACCATCACGGTGGT-AAAAAATTACTCAACAAAG |
| PsarA-sodRBS-6×-His-GG-agrDIII-SO4 | CCC-CCATGG(NcoI)-TTATTCGTGTAATTGAGTTAATT |
| AgrD-1 | AAA-GGTACC(KpnI)-TCCATTTTACTAAGTCACCG |
| AgrD-2 | CTCTCTATTTAAATTATTCGTGATTCATTTTAAGTCCTCCTTA |
| AgrD-3 | TAAGGAGGACTTAAAATGAATCACGAATAATTTAAATAGAGAG |
| AgrD-4 | AAA-GAGCTC(SacI)-TCGGGTATTTCGATACTAAT |
| T1LeaderREV | AAA-GAATTC(EcoRI)-TTAAGCTGCGATGTTACCAATGT |
| T1Leader-AIPREV | AAA-GAATTC(EcoRI)-TTACATTATGAAGTCACAAGT |
| FLAG-GG-MroQSOE1 | CCC-CTGCAG(PstI)-CTGATATTTTTGACTAAACCAA |
| FLAG-GG-MroQSOE2 | ACCACCCTTGTCGTCATCGTCTTTGTAGTCGCTGCTGCCCAT-AAATAATCATCCTCCTAAGGT |
| FLAG-GG-MroQSOE3 | ACAAGATTATGGGCATCATT-ATGGGCAGCAGCGACTACAAAGACGATGACGACAAGGGTGGT |
| FLAG-GG-MroQSOE4 | CCC-GAATTC(EcoRI)-TTATGGAATAAAAATGTGATATA |