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Expression analysis of CTLA-4 in T cells from M. avium subsp. paratuberculosis-infected cattle. The expression of CTLA-4 in CD4+ T cells (CD3+ IgM− CD4+) (a) and CD8+ T cells (CD3+ IgM− CD8+) (b) was analyzed using flow cytometry (uninfected [cattle not infected with M. avium subsp. paratuberculosis], n = 10; infected [M. avium subsp. paratuberculosis-infected cattle], n = 10; n.s., not significant [the P value was obtained using Mann-Whitney U test]).
