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Vacuolar localization of Pib2 is necessary but not sufficient for TORC1 reactivation. (A) Localization of the indicated vacuolar targeting constructs. Vacuoles stained with FM4-64. (B) Rapamycin exposure and recovery assays of Δpib2 cells expressing the indicated targeting constructs. These were performed as in Fig. 1. Images are representative of three experiments.
