TABLE 2.

Oligonucleotides used in PCR^a

Primer	Sequence	Tmb (°C)
RA6OMPA-L	GCAACAGGAGCATTACAAGGTA	50
RA6OMPA-R	CTGTCTTTCATTCTCTCTTTC	50
RAOMPAH1-R	cgctatggatccTTATTTTCTTTTCTT	48
RAOMPAH1A-R	tacggatccTTTTCTTTTCTTTTTTACTAC	48
RAOMPAH1-L	cgcagccatATGGGTTAAAGAATTT	48
RAOMPAF1	GACTGGCAAACTTCAGTAGG	55
RAOMPAF2	TGGTCTTGGTATCCAAGGGG	55
RAOMPAF3	AATAACGGTTGCCCTTGGCC	55
RAOMPAF4	GCTGCTTTAGAAGCTAGAGG	55
RAOMPAR1	CAATGAAGCTGACGCTTGCC	55
RAOMPAR2	GCCCAGGAACTGTAGGACAC	55
RAOMPAR3	GTAGCTTCAGCAGAACCAAC	55
RAOMPAR4	CAACGAGCCATGCTTAGAGGC	55
PETHIS1-3′	CGTCTTCAAGCTCATGTTTG	55
T7	TAATACGACTCACTATAGGG	55
T3B-S	GCGCGCAATTAACCCTCACTAAAG	73

^aUppercase letters correspond to the nucleotides of the ompA sequence; lowercase letters specify nucleotides that were added to create restriction enzyme recognition sites for cloning. Primers pETHIS1-3′ and T7 match the segments flanking the codons of the multiple cloning site in vector pETHIS-1 and were used for verifying the correct fusions of the genes cloned in pETHIS-1. 

^bT_m, annealing temperature.