Fig 2. Expression of the PHO5 gene is negatively affected in RNA exosome mutant cells.
(A) Acid phosphatase induction kinetics in wild-type BMA41 (wt) and corresponding deletion mutant cells for Rrp6 and RNA exosome cofactors upon induction through phosphate starvation. Reported values represent the means and standard deviations of three independent experiments (n = 3). (B) Same as (A), but for W303-derived strains with genomic copy of DIS3 gene deleted but bearing a centromeric plasmid that carries the wild-type copy of DIS3 gene (dis3Δ + pDis3) or its alleles with abolished endonuclease (dis3Δ + pDis3-endo-, D171N) or exonuclease (dis3Δ + pDis3-exo-, D551N) activity.