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. 2022 Oct 10;18(10):e1010432. doi: 10.1371/journal.pgen.1010432

Fig 7. PHO5 AS elongation affects PHO5 gene expression via histone acetylation.

Fig 7

(A) Heatmap of the PHO5 gene locus in wild-type W303 (wt), rpd3Δ, rrp6Δ, and rrp6Δrpd3Δ mutant cells. Snapshot of tilling arrays intensities from [15] at the PHO5 locus for the Watson (W, upper half) and the Crick (C, lower half) strands. Three replicates of each strain are represented. A darker signal depicts a higher score of RNA expression. The red vertical lines represent the inferred coding and non-coding genes boundaries. Below: Levels of PHO5 AS transcript measured by RT-qPCR with the same strains at +Pi conditions. Values were normalized to ACT1 RNA. (B) Acid phosphatase induction kinetics in wild-type W303 (wt) and the corresponding rrp6Δ, rpd3Δ and rrp6Δrpd3Δ cells upon induction through phosphate starvation. Reported values represent the means and standard deviations of three independent experiments (n = 3). (C) As in (B), but for wild-type BY4741 (wt) and the corresponding rrp6Δ, gcn5Δ and rrp6Δgcn5Δ mutant cells.