Table 1. Clinico-pathological characteristics and Nectin-4 expression.

Bonn HNSCC cohort
	Total	Nectin-4 negative	Low Nectin-4	Medium Nectin-4	High Nectin-4	p-value*
All HNSCC cases	n = 159	22 (13.8%)	85 (53.5%)	43 (27.0%)	9 (5.7%)
Patients with clinical data	n = 151
Normal tissue	n = 28	1 (3.6%)	8 (28.6%)	19 (67.9%)	0 (0%)
Gender
Male	126 (83.4%)	13	66	38	9	0.0101
Female	25 (16.6%)	5	17	3	9
Age [mean, standard deviation]	64.4 [9.6]	65.27 [11.84]	64.16 [9.60]	64.45 [10.10]	64.17 [5.19]
Localization
Oral cavity	8 (7.8%)	1	5	0	2	0.9922
Oropharynx	59 (57.8%)	8	29	18	4
Hypopharynx	7 (6.8%)	0	5	2	0
Larynx	28 (27.2%)	2	17	9	0
Unknown	49
Tobacco consumption
Non-smokers	6 (12.5%)	0	0	4	2	0.0011
Smokers	42 (87.5%)	2	27	13	0
Unknown	111
Alcohol consumption
No alcohol	2 (4.5%)	0	2	0	0	0.0672
Occasional	28 (63.6%)	1	14	12	1
Frequent	14 (31.8%)	1	9	3	1
Unknown	115
HPV status (p16)
p16 negative	117 (73.6%)	19	67	26	5	0.0051
p16 positive	42 (26.4%)	3	18	17	4
T-stage
pTis	1 (0.7%)	0	1	0	0	0.4442
pT1	26 (18.6%)	2	17	5	2
pT2	56 (40.0%)	7	27	19	3
pT3	35 (25.0%)	6	22	5	2
pT4	22 (15.7%)	2	11	8	1
Unknown	19
Lymph node involvement
pN0	54 (41.9%)	9	29	14	2	0.5802
pN1	19 (14.7%)	1	10	8	0
pN2	53 (41.1%)	7	28	13	5
pN3	3 (2.3%)	0	3	0	0
Unknown	29
Distant metastases
M0	70 (98.6%)	7	42	18	3	0.6951
M1	1 (1.4%)	0	1	0	0
Unknown	88
Grading
G1	3 (2.2%)	0	1	2	0	0.5452
G2	78 (56.5%)	8	46	18	6
G3	57 (41.3%)	8	29	17	3
Unknown	21

¹Mann–Whitney U test. ²Kruskal-Wallis-test. ^*all Nectin-4 expression categories (negative, low, medium and high) included.