| Thermal cyclers | QuantStudio™5 Real-Time PCR System | Applied Biosystems™ 7500 Fast Dx | CFX96 Touch Real-Time Detection System | No. of Cycles | |||
|---|---|---|---|---|---|---|---|
| RAMP rate | 2.5°C/sec | Fast mode (100%) | 5°C/sec | ||||
| Steps | Temp. (°C) | Time | Temp. (°C) | Time | Temp. (°C) | Time | |
| RT Steps | 55 | 5 min | 55 | 8 min | 55 | 8 min | 1 |
| 95 | 1 min | 95 | 1 min | 95 | 1 min | ||
| PCR Steps | 95 | 7 sec | 95 | 5 sec | 95 | 5 sec | 40 |
| 60 | 30 sec | 60 | 30 sec | 60 | 30 sec | ||
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