Table 3. Comparison of efficiency between standard PCR and Fast PCR.

Thermal cyclers	Targets	Standard PCR (R2)	Fast PCR (R2)
QuantStudio™ 5 Real-Time PCR System	N1	0.9982	0.9819
	N2	0.9951	0.9501
	RP	0.9983	0.9859
Applied Biosystems™ 7500 Fast Dx Real-Time PCR System	N1	0.9936	0.9801
	N2	0.9939	0.9897
	RP	0.9967	0.9959
CFX96 Touch Real-Time PCR detection System	N1	0.9941	0.9971
	N2	0.9902	0.9933
	RP	0.9843	0.9949

To investigate PCR amplification efficiency, linear regression analysis was performed using Ct values generated due to PCR amplification of 10-fold serially diluted solutions containing N1, N2 and RP positive controls having 10000, 1000, 100, 10 and 01 copies each. Finally, R² values were obtained.