Table 5. Precision analysis of fast PCR amplification approach in terms of Ct values.
| Targets | Protocol | QuantStudio™ 5 Real-Time PCR System Standard/ Fast | Applied Biosystems™ 7500 Fast Dx Real-Time PCR System Standard/ Fast | CFX96 Touch Real-Time PCR detection System Standard/ Fast | Significance | |||
|---|---|---|---|---|---|---|---|---|
| Median | Mean ± SD | Median | Mean ± SD | Median | Mean ± SD | |||
| N1 | Standard | 23.43 | 22.48±5.16 | 16.55 | 17.52±2.82 | 16.45 | 16.81±2.88 | NS |
| Fast | 21.76 | 21.71±5.15 | 16.01 | 17.3±4.039 | 16.48 | 17.33±2.94 | ||
| N2 | Standard | 22.25 | 22.23±5.34 | 17.17 | 17.97±2.82 | 16.62 | 17.31±2.73 | NS |
| Fast | 21.29 | 21.22±6.16 | 15.85 | 17.15±4.09 | 16.48 | 17.29±3.13 | ||
| RP | Standard | 25.21 | 25.3±2.4 | 27.26 | 27.36±3.73 | 23.04 | 22.69±4.11 | NS |
| Fast | 24.96 | 24.98±3.07 | 25.62 | 26.64±6.74 | 21.43 | 21.53±3.71 | ||
The numbers indicate Ct values of N1, N2 and RP of 60 COVID-19 positive samples and are presented as median and mean ± SD for Standard PCR and Fast PCR for the mentioned thermal cyclers. For significance level, medians of each target were compared between the Standard PCR and Fast PCR. In addition, mean and median were also compared to see whether the two PCR approaches were identical. NS indicates non-significance (P>0.05).