Skip to main content
. 2022 Oct 20;17(10):e0274908. doi: 10.1371/journal.pone.0274908

Fig 2. ATP affinity and vanadate sensitivity of purified Spf1p in mixed YPL/detergent micelles (0.35% w/w).

Fig 2

A) ATP affinity of Spf1p in YPL/OG micelles (open circles, dotted line with Km = 0.1332 ± 0.0189 mM ATP and Vmax = 0.3065 ± 0.0969 μmol Pi/min/mg) and in YPL/Tween20 micelles (black circles, black line with Km = 0.2977 ± 0.02569 mM ATP and Vmax = 1.072 ± 0.0270 μmol Pi/min/mg). Curve fittings was performed using Michaelies-Menten kinetics. B) Vanadate sensitivity of Spf1p in mixed YPL/OG micelles (open circles, dotted line with Ki = 10.30 ± 0.7471 μM) and YPL/Tween20 (black circles, black line with Ki = 10.30 ± 0.7471 μM). Curve fittings was performed using Substrate inhibition kinetics. All experiments are performed as technical triplicates (n = 3) and is reported as the average value of these with error bars indicating the standard error.