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. 2022 Oct 20;17(10):e0274908. doi: 10.1371/journal.pone.0274908

Fig 6. ATPase activity of Spf1p and M1 mutated Spf1p in mixed YPL/detergent micelles.

Fig 6

A-D) Specific activity in μmol Pi/min/mg at indicated temperatures in absence of detergent (control) or in the presence of 0.35% (w/w) detergent as indicated. Spf1p (black), Spf1p-F202A,F204A,F207A (red) and Spf1p-F202H,F204H,F207H (blue). All experiments are performed as biological replicates and technical triplicates (n = 6) and is reported as the average value of these with error bars indicating the standard error (except cholate in 1A, n = 2 and only average value reported). E-H) The same data plotted as relative activities in relation to the maximal activity observed. Spf1p (black), Spf1p-F202A,F204A,F207A (red), Arrows indicate optimal temperature and error bars indicate the standard error.