Fig 3. Leukocyte infiltration in the plantar tissue after the paw incision.

The percentage of neutrophils (CD11b⁺Ly6G⁺) and Ly6C^high and Ly6C^low monocytes were measured in the plantar tissue collected from BLT1KO and BLT1WT mice at different time points after the incision. A, Representative forward/scatter dot plots showing the percentage of neutrophils. B, Representative forward/scatter dot plots showing percentages of Ly6C^high (red square gate) and Ly6C^low monocyte populations (black square gate). C, The percentage of neutrophils increased and peaked on day 1 in both groups, but there was no significant difference between groups. D, The percentage of Ly6C^high monocytes increased from days 1 to 3 after the incision in both groups. The percentage of Ly6C^high monocytes in BLT1KO mice were more attenuated than that of BLT1WT mice on days 1 and 3 after the incision (*p < 0.05, **p < 0.01 vs. BLT1WT). E, The percentage of Ly6C^low monocytes increased from day 3 after the incision and remained high until day 7 after the incision in both groups. However, there were no significant differences between groups. Data are shown as means ± SEM (n = 5–6 per group). A two-way ANOVA with Bonferroni post hoc tests was used for statistical analysis.