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. 2022 Oct 6;13:982786. doi: 10.3389/fimmu.2022.982786

Figure 5.

Figure 5

β5i/LMP7 activity inhibition controls activation of innate immune responses in C20 cells. (A) Western blot analysis of type I IFN response proteins in C20 cells treated with 200nM of ONX-0914 for indicated time (n=3). (B, C) Quantitative reverse transcription PCR (qRT-PCR) analysis of IFNβ1 (B), and IFN score (C) calculated by interferon stimulated genes (ISGs) median fold change in C20 cells treated with 200nM of ONX-0914 for indicated time (n=5). (D) Western blot analysis of NF-κB pathway proteins in C20 cells treated with 200nM of ONX-0914 for 24h (n=3). (E) qRT-PCR analysis of NF-κB targets in C20 cells treated with 200nM of ONX-0914 for indicated time (n=5). Mean ± SEM; *: P < 0.05, **: P < 0.01, ***: P < 0.001.