TABLE 1.

Summary of the various antibodies used

Controls, cells, or expt	Antibody(-ies)a	Clone(s)	Company
Autofluorescence control	None added
Isotype controls	IgG1-PE, IgG2a-FITC	2T8-2F5, 7T4-1F5	Coulter Corp.
	IgG2b-PE	X39	Becton Dickinson
Color compensation controls	Anti-CD14–FITC, anti-CD4–PE, anti-HLA-DR–TRIb
Monocytes	Anti-CD14–PE	LeuM3	Becton Dickinson
		Mo-2	Coulter Corp.
	Anti-CD14–FITC	MY4	Coulter Corp.
	Anti-CD4–PE	T4	Coulter Corp.
		Q4120	Sigma
	Anti-HLA-DR–FITC	L243	Becton Dickinson
	Anti-HLA-DR–TRI	TU-36	Caltag Laboratories, San Francisco, Calif.
	Combinations: two color, anti-CD14–anti-CD4 and anti-CD14–anti-HLA-DR; three-color, anti-CD14–anti-CD4–anti-HLA-DR
T cells	Anti-CD3–ECD	HIT3A	Coulter Corp.
	Anti-CD3–FITC	Leu4	Becton Dickinson
	Anti-CD3–TRI	S4.1	Caltag Laboratories
	Anti-CD3–Quantum Red	UCHT-1	Sigma
	Anti-CD4–PE	Various clones as described for monocytes
	Anti-CD3–anti-CD4 combination
B cells	Anti-CD19–ECD	HD237	Coulter Corp.
	Anti-CD19–FITC	SJ25-C1	Sigma
		FMC63	Serotec, Mississauga, Ontario, Canada
	Anti-CD20–PE	Leu16	Becton Dickinson
	Anti-HLA-DR	Various clones as described for monocytes
	Combinations: anti-CD19–anti-HLA-DR and/or anti-CD20–anti-HLA-DR
CD4 tagging experiments	Goat anti-mouse Ig–FITC	Not applicable	Biosource International, Camarillo, Calif.

^aTRI and Quantum Red are Cy5-PE tandem dyes. ECD, energy-coupled dye. 

^bColor compensation was performed on whole-blood monocytes. For each fluorochrome, the antibody which would result in the highest-intensity stain was used.