TABLE 10.
Monocyte CD4 tagging experiments: anti-CD4-PE signal
| Isolation no. | Monocyte CD4 expression (mean channel no.) in:
|
||||
|---|---|---|---|---|---|
| Whole blood (baseline)
|
Overnight PBMC cultures
|
||||
| Untagged cultures
|
CD4-tagged cultures (CD4c) | ||||
| Autoa | CD4b | Auto | CD4b | ||
| 19 | 0.640 | 8.02 | 0.551 | 0.846 | 1.68 |
| 24 | 0.560 | 3.74 | 0.365 | 0.485 | 2.47 |
| 25 | 0.269 | 3.09 | 0.253 | 0.688 | 4.52 |
| Meand ± SEM | 4.46 ± 1.46 | 0.283 ± 0.091 | 2.89 ± 0.85e | ||
a
Mean channel number of autofluorescence (FL2 log).
b
Mean channel number of anti-CD4–PE signal.
c
Mean channel number of anti-CD4–PE signal, with MAb added prior to overnight culturing.
d
Corrected for autofluorescence (except for CD4-tagged culture).
e
The difference between the anti-CD4–PE signals for untagged and tagged cultures does not reach statistical difference; P > 0.050.