TABLE 11.

Monocyte CD4 tagging experiments: use of goat anti-mouse Ig–FITC to localize CD4—anti-CD4–PE MAb complex of tagged monocytes

Isolation no.	Monocyte CD4 expression (mean channel no.) in overnight PBMC cultures
	Untagged cultures		CD4-tagged cultures
	Autoa	GaM-FITCb	Auto	GaM-FITC
19	0.357	0.367	0.372	0.367
24	0.510	0.664	0.654	0.898
25	0.505	0.561	0.763	0.717
Meanc ± SEM		0.073 ± 0.042d		0.064 ± 0.091de

^aMean channel number of autofluorescence (FL1 log). 

^bMean channel number of goat anti-mouse Ig (GaM)–FITC signal. 

^cCorrected for autofluorescence. 

^dNo statistically significant binding of goat anti-mouse Ab to monocytes; P > 0.050. 

^eNo statistically significant difference in levels of goat anti-mouse Ab between untagged and tagged cultures; P > 0.050.