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. 2000 Mar;7(2):182–191. doi: 10.1128/cdli.7.2.182-191.2000

TABLE 2.

Monocyte CD4 expression levels before and after overnight or 6 days of culture in RPMI-FBS

Isolation no. Monocyte CD4 expression (mean channel no.) in:
Whole blood (baseline)
Overnight monocyte or PBMC culturesa
6-day monocyte cultures
Autob CD4c Auto CD4 Auto CD4
1 0.265 6.38 0.232 0.228 NDd ND
2 0.216 4.07 0.266 0.405 ND ND
3 0.193 3.60 0.223 0.367 ND ND
4 0.239 2.54 ND ND 0.869 4.64 (63.1%)e
5 0.190 2.90 0.313 0.424 0.998 1.57
6 0.159 2.29 0.198 0.213 0.330 0.603
7 0.232 4.07 ND ND 0.727 5.82 (83.6%)e
8 0.296 4.49 ND ND 0.774 1.24
9 0.266 2.79 ND ND 0.771 2.57
10 0.216 2.97 0.529 0.691 3.95 5.48
11 0.181 2.76 0.296 0.308 2.17 2.46
12 0.218 3.08 0.271 0.313 2.16 3.74
13 0.204 2.54 0.228 0.267 1.53 2.17
14 0.223 2.75 0.192 0.197 2.15 5.56
15 0.235 3.02 0.206 0.222 1.49 2.07
16 0.235 3.10 0.301 0.204 1.44 1.33
17 0.310 2.67 0.358 0.324 1.22 1.27
18 0.276 5.33 0.324 0.200 ND ND
19 0.640 8.02 0.551 0.846 ND ND
20 0.224 4.79 0.705 0.692 ND ND
21 0.291 5.54 0.269 0.225 ND ND
22 0.385 6.61 0.642 0.486 ND ND
23 0.590 9.15 0.457 0.777 ND ND
24 0.560 3.74 0.365 0.485 ND ND
25 0.269 3.09 0.253 0.688 ND ND
26 0.190f 4.02f 0.492 1.02 ND ND
27 0.125f 3.87f 0.269 0.244 ND ND
28 0.302 6.88 0.256 0.426 ND ND
29 0.427 4.63 0.256 0.269 ND ND
30 0.315 4.51 0.234 0.362 ND ND
31 0.284 4.49 0.249 0.264 ND ND
32 0.298 5.09 0.201 0.645 ND ND
33 0.315g 7.45g 0.427g 1.32g ND ND
34 0.459g 2.05g 0.173g 0.820g ND ND
35 0.336g 5.68g 0.148g 0.883g ND ND
Meanh ± SEM 4.04 ± 0.29i 0.159 ± 0.047ij 1.43 ± 0.44ik
a

Isolations 1 to 17, monocyte culture; isolations 18 to 35, PBMC culture. 

b

Mean channel number of autofluorescence (FL2 log). 

c

Mean channel number of anti-CD4–PE signal. 

d

ND, not done. 

e

Increased CD4 expression was observed for a subpopulation of monocytes. 

f

Whole-blood data not available due to Q-Prep malfunction; data from post-Ficoll-Paque PBMC are shown. 

g

Data from the citrate condition of the anticoagulant experiments. 

h

Corrected for autofluorescence. 

i

Statistically significant CD4 expression relative to autofluorescence; P < 0.050. 

j

Statistically significant reduction in CD4 expression relative to whole blood; P < 0.050. 

k

Statistically significant increase in CD4 expression relative to overnight culture; P < 0.050.