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. 2000 Mar;7(2):182–191. doi: 10.1128/cdli.7.2.182-191.2000

TABLE 3.

Monocyte HLA-DR expression levels before and after overnight or 6 days of culture in RPMI-FBS

Isolation no. Monocyte HLA-DR expression in:
Whole blood (baseline)
Overnight monocyte or PBMC culturesa
6-day monocyte cultures
Autob HLA-DRc Autob HLA-DRc Autob HLA-DRc
1 0.235 18.3 0.611 86.4 NDd ND
2 0.618 28.4 0.289 43.6 ND ND
3 0.489 30.4 0.191 51.7 ND ND
4 0.232 26.0 ND ND 4.58 414.7
5 0.622 4.85 0.566 14.9 2.82 37.7
6 0.290 8.17 0.389 13.7 2.85 14.9
7 0.329 15.5 ND ND 5.10 169.3
8 0.255 28.0 ND ND 3.59 129.4
9 0.377 11.8 ND ND 3.46 55.9
10 0.976 12.8 1.49 49.7 13.4 270.7
11 0.265 12.6 0.369 17.7 1.33 38.7
12 0.354 4.66 0.340 20.0 2.03 29.9
13 0.304 4.35 0.295 8.46 1.41 32.7
14 0.358 6.18 0.637 21.4 2.08 33.7
15 0.340 4.60 0.338 8.87 1.37 12.2
16 0.359 8.38 0.514 11.3 1.48 9.01
17 0.480 7.13 0.458 14.6 1.56 36.6
18 0.299 13.3 0.378 30.9 ND ND
19 0.439 15.8 0.353 40.0 ND ND
20 0.244 11.0 0.681 30.0 ND ND
21 0.599 15.1 0.594 48.5 ND ND
22 0.702 18.9 0.895 36.1 ND ND
23 0.630 11.9 0.548 33.6 ND ND
24 0.488 11.6 0.583 45.5 ND ND
25 0.599 6.65 0.505 27.4 ND ND
26 0.205e 26.8e 0.532 191.7 ND ND
27 0.141e 7.97e 0.275 56.5 ND ND
28 0.216 14.0 0.239 81.0 ND ND
29 0.289 5.96 0.447 6.97 ND ND
30 0.447 5.46 0.366 9.54 ND ND
31 0.486 3.17 0.348 2.54 ND ND
32 0.428 4.01 0.305 6.56 ND ND
Meanf ± SEM 10.33 ± 1.13g 35.56 ± 7.08gh 88.45 ± 31.2gi
a

Isolations 1 to 17, monocyte culture; isolations 18 to 32, PBMC culture. 

b

Mean channel number of autofluorescence (FL1 log or FL4 log). 

c

Mean channel number of anti-HLA-DR–FITC or anti-HLA-DR–TRI staining. 

d

ND, not done. 

e

Whole-blood data not available due to Q-Prep malfunction; data from post-Ficoll-Paque PBMC are shown. 

f

Corrected for autofluorescence. 

g

Statistically significant HLA-DR expression relative to autofluorescence; P < 0.050. 

h

Statistically significant increase in HLA-DR expression relative to whole-blood monocytes; P < 0.050. 

i

Increase in HLA-DR expression by day 6 cultured monocytes relative to overnight-cultured monocytes does not reach statistical significance; P > 0.050.