TABLE 4.

Monocyte CD4 expression levels before and after overnight culture in RPMI-FBS, with or without various monocyte/macrophage growth factors, suppressors, and activators

Isolation no.	Monocyte CD4 expression (mean channel no.) in:
	Whole blood (baseline)		Overnight monocyte cultures
			RPMI-FBS		With GM-CSF		With M-CSF		With IL-10		With LPS
	Autoa	CD4b	Auto	CD4	Auto	CD4	Auto	CD4	Auto	CD4	Auto	CD4
1	0.265	6.38	0.232	0.228	0.247	0.235	0.231	0.212	NDc	ND	0.231	0.277
2	0.216	4.07	0.266	0.405	0.286	0.497	0.366	0.574	ND	ND	0.369	0.373
3	0.193	3.60	0.223	0.367	0.243	0.344	0.233	0.349	ND	ND	0.248	0.296
12	0.218	3.08	0.271	0.313	0.362	0.397	ND	ND	0.503	0.525	ND	ND
14	0.223	2.75	0.192	0.197	ND	ND	ND	ND	0.224	0.317	ND	ND
16	0.235	3.10	0.301	0.204	ND	ND	ND	ND	0.358	0.190	ND	ND
Meand ± SEM		3.61 ± 0.58e		0.038 ± 0.031f		0.084 ± 0.048g		0.102 ± 0.066g		−0.018 ± 0.078g		0.033 ± 0.014g

^aMean channel number of autofluorescence (FL2 log). 

^bMean channel number of anti-CD4–PE signal. 

^cND, not done. 

^dCorrected for autofluorescence. 

^eStatistically significant CD4 expression relative to autofluorescence; P < 0.050. 

^fStatistically significant reduction in CD4 expression relative to whole-blood monocytes; P < 0.050. 

^gNo statistically significant difference in CD4 expression between monocytes cultured in RPMI-FBS and cultures supplemented with various factors.