TABLE 5.
Monocyte HLA-DR expression following overnight culture with IL-10
Isolation no. | Monocyte HLA-DR expression (mean channel no.) in overnight monocyte cultures
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---|---|---|---|---|
RPMI-FBS
|
With IL-10
|
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Autoa | HLA-DRb | Auto | HLA-DRc | |
12 | 0.340 | 20.0 | 0.600 | 0.646 (25.0%)d, 10.5 (75.0%)e |
14 | 0.637 | 21.4 | 3.68 | 3.50 |
16 | 0.514 | 10.8 | 0.575 | 0.908 (20.8%)d, 6.14 (79.2%)e |
Meanf ± SEM | 16.9 ± 3.3g | 0.066 ± 0.148d, 5.10 ± 2.92e |
Mean channel number of autofluorescence (FL1 log).
Mean channel number of anti-HLA-DR–FITC signal.
In isolations 12 and 16, two populations were observed with respect to HLA-DR expression.
Low expression.
High expression.
Corrected for autofluorescence.
Statistically significant HLA-DR expression relative to autofluorescence; P < 0.050.