TABLE 7.

Monocyte CD4 expression levels before and after overnight culture in adherent and nonadherent environments

Isolation no.	Monocyte CD4 expression (mean channel no.) in:
	Whole blood (baseline)		Overnight PBMC cultures in:
			Gelatin-fibronectin-coated polystyrene flasks		Uncoated polystyrene flasks		Teflon vials
	Autoa	CD4b	Auto	CD4	Auto	CD4	Auto	CD4
18	0.276	5.33	0.324	0.200	0.440	0.253	NDc	ND
20	0.224	4.79	0.705	0.692	0.566	0.714	ND	ND
21	0.291	5.54	0.269	0.225	0.310	0.289	ND	ND
26	0.190d	4.02d	0.492	1.02	0.352	1.32	0.368	1.21
27	0.125d	3.87d	0.269	0.244	0.220	0.220	0.220	0.279
28	0.302	6.88	0.256	0.426	0.198	0.389	0.237	0.420
Meane ± SEM		4.84 ± 0.43f		0.082 ± 0.098g		0.183 ± 0.166g		0.361 ± 0.243b

^aMean channel number of autofluorescence (FL2 log). 

^bMean channel number of anti-CD4–PE signal. 

^cND, not done. 

^dWhole-blood data not available due to Q-Prep malfunction; data from post-Ficoll-Paque PBMC are shown. 

^eCorrected for autofluorescence. 

^fStatistically significant CD4 expression relative to autofluorescence; P < 0.050. 

^gStatistically significant decrease in CD4 expression relative to whole blood; P < 0.050. 

^hAlthough the reduction in CD4 expression in Teflon-cultured monocytes compared to whole-blood monocytes did not quite reach statistical significance (P value, between 0.060 and 0.050), no statistically significant difference in CD4 expression between the three types of overnight cultures (P > 0.050) was observed.