TABLE 9.

Monocyte CD4 expression levels (as a function of anticoagulant^a) before and after overnight culture in RPMI-FBS

Isolation no.	Monocyte CD4 expression (mean channel no.) in:
	Whole blood (baseline) with:						Overnight PBMC cultures with:
	Citrate		EDTA		Heparin		Citrate		EDTA		Heparin
	Autob	CD4c	Auto	CD4	Auto	CD4	Auto	CD4	Auto	CD4	Auto	CD4
33	0.315	7.45	0.445	3.70	0.456	6.51	0.427	1.32	0.353	0.956	0.334	0.943
34	0.459	2.05	0.408	4.24	0.371	2.61	0.173	0.820	0.140	0.922	0.174	0.954
35	0.336	5.68	0.353	5.79	0.359	3.95	0.148	0.883	0.143	0.721	0.147	0.726
Meand ± SEM		4.69 ± 1.63e		4.17 ± 0.65e		3.96 ± 1.12e		0.758 ± 0.072f		0.654 ± 0.064f		0.656 ± 0.063f

^aFor each isolation, whole blood was collected in three different anticoagulants and assessed for CD4 expression. PBMC were subsequently isolated from each whole-blood–anticoagulant combination, and the overnight PBMC cultures were assessed for monocyte CD4 expression. 

^bMean channel number of autofluorescence (FL2 log). 

^cMean channel number of anti-CD4–PE signal. 

^dCorrected for autofluorescence. 

^eNo statistically significant difference in CD4 expression between any of the whole-blood conditions; P > 0.050. 

^fNo statistically significant difference in CD4 expression between any of the overnight cultures; P > 0.050.