Skip to main content
. 2022 Oct 10;11:e81505. doi: 10.7554/eLife.81505

Figure 5. Loss of presynaptic Rac1 facilitates synaptic vesicle recovery.

Figure 5.

Recovery of single EPSC (EPSCtest) and RRP recovery was measured by two consecutive train stimuli (conditioning stimulus and recovery stimulus) at 500 Hz at varying recovery intervals. (A) Single EPSC recovery. (A1) Representative traces for Rac1+/+ (black) and Rac1−/− (orange) for recovery intervals ranging from 20 ms to 16 s. (A2) Recovery of absolute EPSC amplitudes as a function of recovery interval with a magnification of short intervals (right). (A3) Fractional EPSC recovery as a function of recovery interval. (B) Recovery of the RRP. (B1) Representative recovery traces following a 100 ms and 2 s recovery interval. The conditioning stimulus train is the same as in A1. (B2) Fractional RRP recovery was faster in Rac1−/− compared to Rac1+/+. (C) Recovery of the paired-pulse ratio (PPR) of the first two EPSCs of the recovery train. PPR was consistently lower in Rac1−/− but the difference was emphasized at longer recovery intervals. All data shown in the figure and the detailed results of statistical tests are part of the supplementary file.

Figure 5—source data 1. Excel file containing the data shown in Figure 5 and the results of statistical analysis.