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. 2022 Oct 6;11:e80127. doi: 10.7554/eLife.80127

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© 2022, Yang et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 2. — (A) Subclusters of hemocytes–prohemocytes (PH), monocytic hemocytes (MH), granulocytes (GH) are projected onto two-dimensional t-SNE plots. The numbers in the plots represent the subcluster number. (B) Dot plot showing corresponding expression of cluster marker genes. The color indicates mean expression and dot size represents the percentage of cells within the cluster expressing the marker. Last nine digits of each marker gene are the NCBI GeneID. (C) Expression of cell-cycle regulating genes in the six subtypes. Dot color indicates average expression levels and dot size displays the average percentage of cells with cell cycle controlling genes (Cdk1(ncbi_113818305), CycD(ncbi_113814652), and CycE(ncbi_113822658) for G1; stg(ncbi_113800052), CycA(ncbi_113821735), and CycB(ncbi_113803283) for G2; polo(ncbi_113805901), aurB(ncbi_113827838), and birc5(ncbi_113828653) for M) in each subcluster. (D) A differentiation trajectory of PH, GH, and MH subpopulation using Monocle2 (n=31821). (E) Differentiation trajectory reconstruction with 6 subclusters. PH lineage, GH lineage, and MH lineage were labelled with red, blue, and brown circles respectively. (F) A heatmap showing differentially expressed gene dynamics during hemocyte differentiation process. (G) Spline plots showing the expression dynamics of Fli1, c-Rel, and MAF. Imaginary line, monocytic hemocyte lineage; Full line, granulocyte lineage.

Figure 2—figure supplement 1. — (A) Subclusters of hemocytes–prohemocytes (PH), monocytic hemocytes (MH), granulocytes (GH) are projected onto two-dimensional t-SNE plots. The numbers in the plots represent the subcluster number. (B) Dot plot showing corresponding expression of cluster marker genes. The color indicates mean expression and dot size represents the percentage of cells within the cluster expressing the marker. Last nine digits of each marker gene are the NCBI GeneID. (C) Expression of cell-cycle regulating genes in the six subtypes. Dot color indicates average expression levels and dot size displays the average percentage of cells with cell cycle controlling genes (Cdk1(ncbi_113818305), CycD(ncbi_113814652), and CycE(ncbi_113822658) for G1; stg(ncbi_113800052), CycA(ncbi_113821735), and CycB(ncbi_113803283) for G2; polo(ncbi_113805901), aurB(ncbi_113827838), and birc5(ncbi_113828653) for M) in each subcluster. (D) A differentiation trajectory of PH, GH, and MH subpopulation using Monocle2 (n=31821). (E) Differentiation trajectory reconstruction with 6 subclusters. PH lineage, GH lineage, and MH lineage were labelled with red, blue, and brown circles respectively. (F) A heatmap showing differentially expressed gene dynamics during hemocyte differentiation process. (G) Spline plots showing the expression dynamics of Fli1, c-Rel, and MAF. Imaginary line, monocytic hemocyte lineage; Full line, granulocyte lineage.