TABLE 3.

Somatic indices and IGF-1 plasma levels of gilthead sea bream juveniles fed with a high-fat diet in the absence (HF) or presence (HF + HT) of hydroxytyrosol (0.52 g HT/kg feed), at a standard (ST) (3% biomass/tank) or restricted ration (RE) (40% daily reduction) for 8 weeks.

	HF_ST	HF_RE	HF + HT_ST	HF + HT_RE	D	R	D*R
FBW (g)	195.63 ± 13.54	168.65 ± 6.81	194.00 ± 7.24	163.96 ± 1.40	0.430	0.017	0.901
BL (cm)	19.04 ± 0.40	18.34 ± 0.09	19.31 ± 0.17	18.09 ± 0.34	0.977	0.009	0.381
CF	2.85 ± 0.04	2.75 ± 0.06	2.78 ± 0.05	2.66 ± 0.06	0.203	0.085	0.794
FCR	1.66 ± 0.25	0.99 ± 0.06	1.68 ± 0.15	1.08 ± 0.10	0.744	0.004	0.822
Liver (g)	2.42 ± 0.14	2.08 ± 0.20	2.56 ± 0.21	1.96 ± 0.20	0.973	0.036	0.523
HSI (%)	1.23 ± 0.05	1.20 ± 0.09	1.29 ± 0.003	1.23 ± 0.06	0.466	0.475	0,859
IGF-1 (ng/ml)	3.88 ± 0.13a	4.70 ± 0.12b,c	4.97 ± 0.10c	4.44 ± 0.11b	0.001	0.225	<0.001

Data are shown as Mean ± SEM (n = 3 tanks, except for IGF-1 plasma levels n = 10 fish). Statistical differences are indicated in 3 components: diet (D), ration (R) and interaction (D*R), using two-way ANOVA (p < 0.05). When the interaction between the two factors (D*R) was significant, comparisons among groups were analyzed by a Tukey’s post-hoc test and, significant differences are indicated by different letters (p < 0.05). FBW, final body weight; BL, body length; CF, condition factor (FBW/BL³) $\times$ 100; FCR, feed conversion ratio (g total feed intake/(FBW- initial BW); HSI, hepatosomatic index (liver weight/FBW) $\times$ 100.