Table 1.
A. thaliana lines used in this study
| Name | Accession Number | Source or reference |
|---|---|---|
| Col-0 | ||
| cfs1-2 | At3g43230 | SALK_024058; Sutipatanasomboon et al. (2017) Sci. Rep. |
| cfs2-1 | At1g29800 | SALK_02775; this study |
| atg5-1 | At5g17290 | SAIL_129B07L; Thompson et al. (2005) Plant Physiol. |
| vps23.1-3 | At3g12400 | SAIL_1233E07_2-4; Nagel et al. (2017) PNAS. |
| vps23.2-1 | At5g13860 | SAIL_237G05_3-4; Nagel et al. (2017) PNAS. |
| cfs1-2 × cfs2-1 | At3g43230 (CFS1); At1g29800 (CFS2) | This study |
| pUBQ::mCherry | Geldner et al. (2009) Plant J. | |
| pUBQ::mCherry | This study | |
| pUBQ::GFP-ATG8A | At4g21980 | Munch et al. (2015) The Plant Cell. |
| pUBQ::GFP-ATG8A × cfs1-2 | At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-ATG8E | At2g45170 | Hu et al. (2020) J. Integr. Plant Biol. |
| pUBQ::mCherry-ATG8E × cfs1-2 | At2g45170 (ATG8E); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-ATG8E × atg5-1 | At2g45170 (ATG8E); At5g17290 (ATG5) | This study |
| pCFS1::gCFS1 in cfs1-2 | At3g43230 | Sutipatanasomboon et al. (2017) Sci Rep. |
| pUBQ::mCherry-CFS1 in cfs1-2 | At3g43230 | This study |
| pUBQ::mCherry-CFS1AIM in cfs1-2 | At3g43230 | This study |
| pUBQ::GFP-CFS1 in cfs1-2 | At3g43230 | This study |
| pUBQ::mScarlett-CFS2 in pUBQ::GFP-CFS1 in cfs1-2 | At1g29800 (CFS2); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-CFS1FYVE in pUBQ::GFP-ATG8A × cfs1-2 | At3g43230 (CFS1); At4g21980 (ATG8A) | This study |
| pUBQ::mCherry-CFS1SYLF in pUBQ::GFP-ATG8A × cfs1-2 | At3g43230 (CFS1); At4g21980 (ATG8A) | This study |
| pUBQ::mCherry-CFS1tri in pUBQ::GFP-ATG8A × cfs1-2 | At3g43230 (CFS1); At4g21980 (ATG8A) | This study |
| pUBQ::mCherry-CFS1PSAPP in cfs1-2 | At3g43230 | This study |
| pUBQ::mCherry-CFS1PSAPP in pUBQ::GFP-ATG8A × cfs1-2 | At3g43230 (CFS1); At4g21980 (ATG8A) | This study |
| pUBQ::mCherry-CFS1AIM+PSAPP in cfs1-2 | At3g43230 | This study |
| pUBQ::mCherry-CFS1AIM+PSAPP in pUBQ::GFP-ATG8A × cfs1-2 | At3g43230 (CFS1); At4g21980 (ATG8A) | This study |
| pUBQ::GFP-VPS23A in pUBQ::mCherry-CFS1 in cfs1-2 | At3g12400 (VPS23A); At3g43230 (CFS1) | This study |
| pUBQ::GFP-VPS23A in pUBQ::mCherry-CFS1PSAPP in cfs1-2 | At3g12400 (VPS23A); At3g43230 (CFS1) | This study |
| pUBQ::VPS23A-mTurquoise2 in pUBQ::GFP-ATG8A × pUBQ::mCherry-CFS1 in cfs1-2 | At3g12400 (VPS23A); At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| pUBQ::VPS23A-mTurquoise2 in pUBQ::mCherry-CFS1PSAPP in pUBQ::GFP-ATG8A × cfs1-2 | At3g12400 (VPS23A); At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-MpCFS1 in pUBQ::GFP-ATG8A × cfs1-2 | Mp3g11370 (CFS1); At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| p35S::GFP-FREE1 in pUBQ::mCherry-CFS1 in cfs1-2 | At1g20110 (FREE1); At3g43230 (CFS1) | This study |
| atg5-1 × pUBQ::mCherry-CFS1 in cfs1-2 | At5g17290 (ATG5); At3g43230 (CFS1) | This study |
| pUBQ::GFP-ATG8A × pUBQ::mCherry-CFS1 in cfs1-2 | At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| pUBQ::GFP-ATG8A × pUBQ::mCherry-CFS1AIM in cfs1-2 | At4g21980 (ATG8A); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-ATG8E × pUBQ::GFP-CFS1 in cfs1-2 | At2g45170 (ATG8E); At3g43230 (CFS1) | This study |
| pVPS3::mGFP-VPS3 | At1g22860 | Takemoto et al. (2018) PNAS. |
| pVPS3::mGFP-VPS3 × pUBQ::mCherry-CFS1 in cfs1-2 | At1g22860 (VPS3); At3g43230 (CFS1) | This study |
| pVPS39::VPS39-mGFP | At4g36630 | Takemoto et al. (2018) PNAS. |
| pVPS39::VPS39-mGFP × pUBQ::mCherry-CFS1 in cfs1-2 | At4g36630 (VPS39); At3g43230 (CFS1) | This study |
| pUBQ::YFP-VAMP711 | At4g32150 | Geldner et al. (2009) Plant J. |
| pUBQ::YFP-VAMP711 × pUBQ::mCherry-CFS1 in cfs1-2 | At4g32150 (VAMP711); At3g43230 (CFS1) | This study |
| p35S::NAG1-EGFP | At4g38240 | Grebe et al. (2003) Curr Biol. |
| p35S::NAG1-EGFP × pUBQ::mCherry-CFS1 in cfs1-2 | At4g38240(NAG1); At3g43230 (CFS1) | This study |
| pa1::VHA-a1-GFP | At2g28520 | Dettmer et al. (2006) The Plant Cell. |
| pa1::VHA-a1-GFP × pUBQ::mCherry-CFS1 in cfs1-2 | At2g28520 (VHA-a1); At3g43230 (CFS1) | This study |
| pRPS5a::GFP-ARA7 | At4g19640 | Richter et al. (2007) Nature. |
| pRPS5a::GFP-ARA7 × pUBQ::mCherry-CFS1 in cfs1-2 | At4g19640 (ARA7); At3g43230 (CFS1) | This study |
| pPIN::PIN2-GFP | At5g57090 | Xu and Scheres (2005) The Plant Cell. |
| pPIN2::PIN2-GFP × cfs1-2 | At5g57090 (PIN2); At3g43230 (CFS1) | This study |
| pNBR1::TagRFP-NBR1 | At4g24690 | Provided by Alyona Minina |
| pNBR1::NBR1-GFP | At4g24690 | Hafrén et al. (2017) PNAS. |
| pNBR1::NBR1-GFP × pUBQ::mCherry-CFS1 in cfs1-2 | At4g24690 (NBR1); At3g43230 (CFS1) | This study |
| pUBQ::mCherry-ATG8E × pNBR1::NBR1-GFP | At2g45170 (ATG8E); At4g24691 (NBR1) | This study |
| pUBQ::GFP-ATG11 | At4g30790 | Li et al. (2014) The Plant Cell. |
| pUBQ::GFP-ATG11 × pUBQ::mCherry-CFS1 in cfs1-2 | At4g30790 (ATG11); At3g43230 (CFS1) | This study |
| pALIX::GFP-3Gly-ALIX | At1g15130 | Cardona-López et al. (2015) The Plant Cell. |
| pALIX::GFP-3Gly-ALIX × pUBQ::mCherry-CFS1 in cfs1-2 | At1g15130 (ALIX); At3g43230 (CFS1) | This study |
| pVPS23A::VPS23A-TagRFP | At3g12400 | Nagel et al. (2017) PNAS. |
| pVPS23A::VPS23A-TagRFP × pUBQ::GFP-CFS1 in cfs1-2 | At3g12400 (VPS23A); At3g43230 (CFS1) | This study |
| pVPS23A::VPS23A-TagRFP × pUBQ::GFP-ATG8A | At3g12400 (VPS23A); At4g21980 (ATG8A) | This study |
| pUBQ::GFP-ATG8A × vps23.1-3 | At4g21980 (ATG8A); At3g12400 (VPS23A) | This study |
| pUBQ::GFP-ATG8A × vps23.2-1 | At4g21980 (ATG8A); At3g13860 (VPS23B) | This study |