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. 2022 Oct 19;221(12):e202203139. doi: 10.1083/jcb.202203139

Table 3.

Primer and synthetic sequences used in this study for genotyping, cloning, and mutagenesis

Name Sequence Additional information Source or reference
LBb1.3 (SAIL) 5′-ATT​TTG​CCG​ATT​TCG​GAA​C-3′ For genotyping SIGnAL. 2017.
cfs1-2 (LP)  5′-GCT​CGA​CTA​AGA​ACA​GCA​TGC-3′ For genotyping SIGnAL. 2017.
cfs1-2 (RP)  5′-TAC​ATG​GGT​TTG​GAT​GAG​CG-3′ For genotyping This study
cfs2-1 (LP) 5′-TGG​TGA​GTT​TTA​CGC​TTA​CCG-3′ For genotyping SIGnAL. 2017.
cfs2-1 (RP) 5′-TTG​GTC​CAT​CAA​ATA​AGG​CTG-3′ For genotyping SIGnAL. 2017.
LB3 (SALK) 5′-AAT​TTC​ATA​ACC​AAT​CTC​GAT​ACA​C-3′ For genotyping Nagel et al. (2017) PNAS.
vps23.1-3 (LP) 5′-AAC​TTT​GGA​CTT​TGG​AAC​ATG​TCC​ACT​CCT​TAC​A-3′ For genotyping Nagel et al. (2017) PNAS.
vps23.1-3 (RP) 5′-AGG​AAC​CAT​CAT​CAC​ACG​CAT​AGC​CAC​AG-3′ For genotyping Nagel et al. (2017) PNAS.
vps23.2-1 (LP) 5′-TCG​TCA​TCT​GTC​GTC​GTC​TTC​AAG-3′ For genotyping Nagel et al. (2017) PNAS.
vps23.2-1 (RP) 5′-GTA​TCA​CGA​ATG​CAA​CCT​AGC​TGC​AAT​GGA​AG-3′ For genotyping Nagel et al. (2017) PNAS.
CFS1_part1_fwd 5′-AAC​AGG​TCT​CAG​GCT​CTA​TGG​CTA​CTC​TCA​ACG​GAA​A-3′ For cloning CDS, part 1 This study
CFS1_part1_rev 3′-AAC​AGG​TCT​CAC​GAG​ATA​TTA​CCA​GAC​CAG​T-5′ For cloning CDS, part 1 This study
CFS1_part2_fwd 5′-AAC​AGG​TCT​CTC​TCG​TAG​ACC​AGA​CGG​GTC​ATG​G-3′ For cloning CDS, part 2 This study
CFS1_part2_rev 3′-AAC​AGG​TCT​CAC​TGA​CGG​GCG​CAA​ACG​AGC​ATA-5′ For cloning CDS, part 2 This study
CFS2_part1_fwd 5′-ACC​CTC​TGC​CAT​CTC​TTC​ATT​TGG​T-3′ For cloning CDS, part 1 This study
CFS2_part1_rev 3′-AAC​AGG​TCT​CAG​GGT​GGT​GAC​CAG​GAG​CCA​TCA​TCT-5′ For cloning CDS, part 1 This study
CFS2_part2_fwd 5′-TCC​TGG​TCT​CCA​CCC​TCT​GC-3′ For cloning CDS, part 2 This study
CFS2_part2_rev 3′-AAC​AGG​TCT​CAC​TGA​GTC​TTC​AGA​CAA​TGG​AGA​AAT​TGC​CGG​TGA​CCG​AAC​AGT​TTC​A-5′ For cloning CDS, part 2 This study
MpCFS1 5′-AAC​AGG​TCT​CAG​GCT​CTA​TGG​AGG​AGA​ATG​AGT​ACA​ACG​GAC​TCT​GCC​GGA​CAG​ATA​CCG​TCG​AAG​AGG​AGA​AAT​ATT​CTG​AGA​AAA​GTG​GTC​GAT​ACA​AGG​GTG​TCA​TTC​GTG​ATG​AGA​AAC​CCA​CTT​GTG​CTG​TCT​GTA​TGG​AAG​TTC​TAG​GAA​CTC​ATG​GTG​GGC​CTG​CAT​CTC​TCG​CCT​GCG​GCC​ACA​ATG​GAT​GTC​TCG​AGT​GCT​TAC​AAC​AGG​TCC​AGA​TGC​ACT​CGA​ACA​TGC​CTG​TCT​GTC​CTC​TTT​GTC​GTA​CAC​CCT​TTG​ATG​GAG​ATA​TCA​ATT​TGG​CCC​CGA​ACT​TAG​ACT​TAC​GGG​CTG​CAC​TCG​AGT​GTG​CCG​AAC​AAG​CCG​CCG​CAG​CTG​CGC​GAG​CTG​CCG​AGG​ATC​GGA​GGA​TGG​TTG​CTG​TGT​ACA​ATT​CTA​AGC​AGA​TTG​TGA​AGC​CGA​GCA​GAG​ATA​AAA​CCA​CCA​GGT​CAC​ACC​AAC​AGC​GAT​TCA​TCT​ATG​ATT​TGC​CCT​ATC​CTG​AGG​AAA​CAG​GAC​CTT​GGA​TTC​CGG​TGT​CAA​TTC​CGC​CCA​AGG​GCT​CGA​AGG​ACT​TTG​ATG​GGC​CTG​ACA​TGG​GCA​GTA​TAT​ATG​GAG​ATG​TTT​TTC​CGG​AAC​CCT​TGA​TGA​CAG​TGG​GTG​TCC​GAC​AAG​GGG​CTC​CGG​ACA​TCA​ACC​TTT​GGA​ATG​TTT​TGA​GTG​GAT​TTC​TTG​CAA​TCA​TAT​CGG​GAA​GGG​GTG​GTA​CAC​AAA​GCC​AGA​ATT​TCC​ACT​CAC​AGT​CCG​GTT​ATT​CTG​ATA​GTA​GAA​CAT​CTA​CGT​ACT​ACC​GAG​AAA​GTT​CTG​GTC​ATG​GAA​GAT​CTA​ACA​GCA​ATT​CAT​GGA​GTG​GGA​ATT​TAC​ACA​GGC​CAA​GTG​CAC​CTC​CAC​TGC​TCC​TTG​GCA​CTA​ATG​ATT​CCG​ATG​TCA​GCC​TCA​TAC​GAG​CTA​TTC​TCG​AAG​CAG​AGC​CCC​CGG​ACT​GGA​TGC​CTG​ACA​GTT​CGG​CCC​TTT​CCT​GTA​TGC​TCT​GTG​GCG​CTG​CCT​TTA​GGG​CAA​TAA​CTT​GTG​GAA​GAC​ATC​ATT​GTC​GGT​TCT​GCG​GGG​GAA​TTT​TTT​GTA​GGA​GAT​GCT​CTT​CCG​GGA​GAT​GTC​TTT​TAC​CTG​TGA​AGT​TCC​GGG​ACA​GGG​ACC​CGA​AAA​GAG​TTT​GCG​ATA​CCT​GCT​GGG​AGA​GAC​TGG​AAC​CTG​TAC​AGA​AGA​ATC​TTG​CCG​ACC​GAG​TTA​GTA​ATG​CAG​CAC​AAA​TCG​CTC​TAC​ATG​ATG​TGA​CTG​ACT​TCA​GCT​GTA​TGA​CAG​CAT​GGA​TAA​ACA​GTC​CAC​TTG​GGC​TTT​CGA​TGG​AGC​AAG​AAA​TTT​TTA​AGG​CAA​CAA​ATG​CCT​TGC​GTA​GCT​ATT​TGG​AGA​TTG​GAG​TAC​TCA​AAC​CAG​AAA​GAT​CCA​TCC​CCG​ACG​CTG​TTC​TGA​AAG​GAG​CCT​GTG​GCT​TGG​CAA​TCA​TCA​CAG​TGT​TCA​AGG​CCG​GGT​TTA​TGA​TGA​CCT​ACA​AAT​TTG​GTA​CGG​GAT​TGG​TTG​TTT​CAC​GGA​GGA​GAG​ATG​GGT​CCT​GGT​CTG​CAC​CAT​CAG​CTA​TCG​CAT​CGT​GTG​GTC​TGG​GAT​GGG​GAG​CTC​AGG​CTG​GTG​GGG​AGC​TGA​CTG​ATT​TCA​TCA​TAG​TCC​TAA​GGA​CAG​AAG​AAG​CTG​TGA​AGG​CCT​TTA​GTG​GGA​GAG​TAC​ATT​TGT​CAG​TTG​GTG​CCG​GCC​TAA​GCG​TCG​CTG​CCG​GAC​CTG​TTG​GAA​GAG​CTG​CGG​AAG​CAG​ATC​TTA​GAG​CTG​GTG​ATG​GGG​GTA​CTG​CAG​CTT​GTT​ACA​CTT​ACA​GTC​GAA​GCA​AAG​GAG​CGT​TCG​TCG​GAT​GTT​CAA​TCG​AAG​GCA​ATG​TTG​TGG​CGA​CAC​GGT​CCT​CCG​CCA​ACA​TGC​GCT​TTT​ATG​GAG​AAT​CTG​GTG​TCA​CTG​CAG​TCG​ACA​TTC​TTC​TTG​GAG​ACG​TTC​CAA​GAC​CTC​GGG​CTG​CAG​CTC​CGC​TTT​ATA​GTG​CTT​TGG​ATG​AAC​TTT​TTG​TAA​AGG​TGG​ACA​CAT​CAG​TGA​GAC​CTG​TT-3′ For cloning CDS This study
MpATG8A_fwd 5′-AAC​AGG​TCT​CAG​GCT​CCA​TGG​GAG​AGA​GCG​AGT​TTC​AAT-3′ For cloning CDS This study
MpATG8A_rev 3′-AAC​AGG​TCT​CAC​TGA​TCA​GCC​AAC​GAG​GGC​GAC-5′ For cloning CDS This study
MpATG8B_fwd 5′-AAC​AGG​TCT​CAG​GCT​CCA​TGA​CGG​GGA​AGA​GGA​GTT​C-3′ For cloning CDS This study
MpATG8B_rev 3′-AAC​AGG​TCT​CAC​TGA​CTA​AAC​CAG​AGG​AAG​GCA​ATT​G-5′ For cloning CDS This study
gRNA_MpCFS1_1_fwd 5′-CTC​GAA​GTT​CTA​GGA​ACT​CAT​GGT-3′ For CRISPR/Cas9 This study
gRNA_MpCFS1_1_rev 5′-AAA​CAC​CAT​GAG​TTC​CTA​GAA​CTT-3′ For CRISPR/Cas9 This study
gRNA_MpCFS1_2_fwd 5′-CTC​GAC​TTC​AGC​TGT​ATG​ACA​GCA-3′ For CRISPR/Cas9 This study
gRNA_MpCFS1_2_rev 5′-AAA​CTG​CTG​TCA​TAC​AGC​TGA​AGT-3′ For CRISPR/Cas9 This study
CFS1_mutAIM_fwd 5′-TGG​ACT​TGC​TCA​AGA​GGA​GCG​TTG​AAT​GCC​CCT​GTT​GGT​CTA​TCC​AT-3′ For site-directed mutagenesis PrimerX. 2017.
CFS1_mutAIM_rev 3′-ATG​GAT​AGA​CCA​ACA​GGG​GCA​TTC​AAC​GCT​CCT​CTT​GAG​CAA​GTC​CA-5′ For site-directed mutagenesis PrimerX. 2017.
CFS1_mutFYVE_fwd 5′-GGT​GCT​CAT​GCT​TGT​GCT​TTC​TGC​GGA​GGG​ATA​TTT​TGT​AGA-3′ For site-directed mutagenesis PrimerX. 2019.
CFS1_mutFYVE_rev 3′-GAA​AGC​ACA​AGC​ATG​AGC​ACC​ACA​CGT​AAT​TGC​AGT​AAA​AGG-5′ For site-directed mutagenesis PrimerX. 2019.
CFS1_mutSYLF 5′-AGT​CTC​CAT​GGA​AGA​TGA​GAT​ATA​CGC​AGC​TGC​TAA​TAC​GTT​GGC​AGG​TTA​CTG​CCA​GGT​AGC​AAG​ACT​AGA​CCC​TGA​GAA​ATC​CAT​ACC​GCA​TGC​TGT​TCT​TAG​TCG​AGC​TAA​AGG​TTT​GGC​AAT​CAT​AAC​GGT​TGC​CGC​TGC​TGG​GGC​TTT​ACT​GTC​ATA​CAA​ACT​CGG​GAC​TGG​TCT​GGT​AAT​ATC​TCG​TAG​ACC​AGA​CGG​GTC​ATG​GTC​TGC​TCC​ATC​TGC​CAT​ACT​ATC​AGT​AGG​TCT​TGG​ATG​GGG​TGC​ACA​GAT​TGG​AGG​TGA​GTT​GAT​GGA​CTT​CAT​AAT​AGT​GCT​TCA​TGA​TGT​GAA​AGC​CGT​GAA​GAC​ATT​CTG​CAG​CAG​AAT​GCA​CTT​CTC​TCT​AGG​CGC​GGG​ATG​CAG​TGC​AGC​AGC​AGG​GCC​TAT​CGG​GAG​AGT​GTT​GGA​GGC​TGA​CCT​TCG​AGC​TGG​TGA​CAA​AGG​CTC​TGG​TGT​CTG​CTA​TAC​TTA​CAG​CCG​CAG​CAA​AGG​GGC​ATT​TGT​GGG​AGT​ATC​TCT​AGA​GAG​ACT-3′ For mutagenesis This study
CFS1_mutPSAPP_fwd 5′-CAT​TCG​TCT​GTT​TAC​ATA​GCG​GCT​GCT​GCG​GCT​CTA​CTT​GAA​CCT​AGT​G-3′ For site-directed mutagenesis PrimerX. 2018.
CFS1_mutPSAPP_rev 3′-CAC​TAG​GTT​CAA​GTA​GAG​CCG​CAG​CAG​CCG​CTA​TGT​AAA​CAG​ACG​AAT​G-5′ For site-directed mutagenesis PrimerX. 2018.
VPS23A_fwd 5′-AAC​AGG​TCT​CAG​GCT​CTA​TGG​TTC​CCC​CGC​CGT​CTA​ATC​C-3′ For cloning CDS, part 1 This study
VPS23A-Bsa1_rev 3′-AAC​AGG​TCT​CAG​AAA​CGA​GGC​CAG​AAG​GAG​TGA​CAT-5′ For cloning CDS, part 1 This study
VPS23A-Bsa1_fwd 5′-AAC​AGG​TCT​CAT​TTC​TCT​TCC​GTA​CCT​TCA​GAA​TTG​G-3′ For cloning CDS, part 2 This study
VPS23A_rev 3′-AAC​AGG​TCT​CAC​TGA​TGA​ATG​TAA​CCT​ACC​TGC​GAT​GGC​TG-5′ For cloning CDS, part 2 This study