Figure 3.

DNMT1 regulated RAI2 expression by regulating RAI2 DNA methylation. (a) After DNMT1 knockdown or overexpression, RAI2 promoter methylation was detected by methylation-specific PCR (MSP). (b) After the knockdown or overexpression of the lncRNA KIF9-AS1, MSP revealed RAI2 promoter methylation. (c) Chromatin immunoprecipitation (ChIP) was performed to measure the extent of the interaction between DNMT1 and the RAI2 promoter. (d and e) qRT–PCR and western blot analyses of RAI2 mRNA and protein expression, respectively. The data are expressed as the means ± SD. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.