Figure 6.

Knocking down lncRNA KIF9-AS1 expression inhibited subcutaneous HCC tumor formation in nude mice. A subcutaneous tumor-forming model in nude mice was established by injecting Huh-7 cells transfected with sh-NC or sh-KIF9-AS1. (a–c) Tumor volume, size, and weight in the nude mice. (d) Ki67 expression was determined by immunohistochemistry (IHC). (e) The expression of KIF9-AS1, DNMT1, and RAI2 was measured by qRT–PCR. (f) Western blot analysis was performed to detect DNMT1 and RAI2 expression. The data are expressed as the means ± SD, n = 8. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.