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. 2022 Oct 13;2022:3017807. doi: 10.1155/2022/3017807

Figure 3.

Figure 3

TUNEL/DAPI analysis of apoptosis induced by 4-carbomethoxyl-10-epigyrosanoldie E treatment in Ca9-22 and Cal-27 cells. Ca9-22 and Cal-27 cells were treated with DMSO or 4-carbomethoxyl-10-epigyrosanoldie E at final concentrations of 10, 15, and 20 μM for 24 h. Cells were harvested for DAPI and TUNEL staining as described in “Materials and Methods.” Scale bar = 50 μm.