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Western blotting analysis of autophagy-related proteins in Ca9-22 and Cal-27 cells treated with 4-carbomethoxyl-10-epigyrosanoldie E. (a) Associated proteins, including Beclin-1, LC3-I, LC3-II, Atg3, Atg5, Atg7, Atg12, and Atg16, were analyzed using Western blotting analysis. (b) The effects of 3-MA application (autophagic inhibitor; 20 μM) prior to treatment with 4-carbomethoxyl-10-epigyrosanoldie E for 24 h on were LC3-I and LC3-II assessed.
