Table 1.
Gene | Size | Polymerase chain reaction protocol | Sequence of gene |
---|---|---|---|
UidA | 147 bp | Initial denaturation was carried out for 7 min at 95°C followed by total 35 cycles of 30 sec each at 95°C; annealing for 30 sec at 55°C; extension for 30 sec at 72°C, followed by final extension at 72°C, for 7 min | F-TGGTAATTACCGAC GAAAACGGC R-ACGCGTG GTTACAGTCTTGCG |
| |||
blaCTX-M-15 | 996 bp | Initial denaturation for 4 min at 96°C followed by total 34 cycles of 20 sec each at 95°C; annealing for 20 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 5 min | F-CACACGTGGAATTTAGGGACT R-GCCGTCTAAGGCGATAAACA |
| |||
tetB | 634 bp | Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min | F-CCTCAGCTTCTCAACGCGTG R-GCACCTTGCTGATGACTCTT |
| |||
TMP-SMX dfrA1 | 367 bp | Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 45°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min | F-GGAGTGCCAAAGGTGAACAGC R-GAGGCGAAGTCTTGGGTAAAAAC |
| |||
PAP | Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, 1 min at 72°C followed by final extension of 5 min at 72°C | F-AACCTGGCTTACGCAACTGTACCC GT R-CTG CAA AAT CAT GGA T |
|
| |||
Heat-labile toxin | Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, and 1 min at 72°C and final extension for 5 min at 72°C | F-GCACACGGAGCTCCTCAGTC R-TCCTTCATCCTT TCA ATG GCT TT |