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. 2022 Oct 13;2022:6495044. doi: 10.1155/2022/6495044

Table 1.

Details of primers and their respective polymerase chain reaction conditions.

Gene Size Polymerase chain reaction protocol Sequence of gene
UidA 147 bp Initial denaturation was carried out for 7 min at 95°C followed by total 35 cycles of 30 sec each at 95°C; annealing for 30 sec at 55°C; extension for 30 sec at 72°C, followed by final extension at 72°C, for 7 min F-TGGTAATTACCGAC GAAAACGGC
R-ACGCGTG GTTACAGTCTTGCG

blaCTX-M-15 996 bp Initial denaturation for 4 min at 96°C followed by total 34 cycles of 20 sec each at 95°C; annealing for 20 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 5 min F-CACACGTGGAATTTAGGGACT
R-GCCGTCTAAGGCGATAAACA

tetB 634 bp Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 56°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min F-CCTCAGCTTCTCAACGCGTG
R-GCACCTTGCTGATGACTCTT

TMP-SMX dfrA1 367 bp Initial denaturation for 3 min at 95°C followed by total 35 cycles of 1 min each at 94°C; annealing for 90 sec at 45°C; 60 sec at 72°C, followed by final extension at 72°C, for 8 min F-GGAGTGCCAAAGGTGAACAGC
R-GAGGCGAAGTCTTGGGTAAAAAC

PAP Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, 1 min at 72°C followed by final extension of 5 min at 72°C F-AACCTGGCTTACGCAACTGTACCC GT
R-CTG CAA AAT CAT GGA T

Heat-labile toxin Initial denaturing 3 min at 94°C, 35 cycles of 1 min at 94°C, 1 min at 58°C, and 1 min at 72°C and final extension for 5 min at 72°C F-GCACACGGAGCTCCTCAGTC
R-TCCTTCATCCTT TCA ATG GCT TT