Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 20;12:17571. doi: 10.1038/s41598-022-22295-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Hakai controls lipid accumulation via FASN. (a) Hakai expression in HCT116 cells transfected with Flag-Hakai was analyzed by Western blot analysis and (b) the effect on lipid accumulation by staining with Oil Red is shown together with quantification of cell staining (right panel). Scale bar 125 μm. (c) HCT116 cells transfected with siRNA-Hakai2 or siRNA control in presence of absence of siRNA-FASN, analyzed by Western blot analysis and (d) staining with Oil Red together with quantification of cell staining results is. (b and d) Five pictures of each sample were taken and quantified. Values are means ± SD of staining intensity signal scoring per area. Quantification and calibration of the images were performed with Image Analysis Toolbox software for Image J. Kruskal–Wallis with Tukey correction test analyses show statistical differences between overexpression/silencing of Hakai or FASN and controls (*p < 0.05; **p < 0,01; ***p < 0,001; ****P < 0.0001). Scale bar 50 μm. (e) Immunohistochemistry staining (letf panel) and quantification (right panel) of FASN in mouse samples of the AOM/DSS Colitis-associated colorectal cancer model (inflamed epithelium and tumours, healthy mice, n = 7; AOM/DSS-treated mice, n = 14). Immunohistochemistry staining and quantification were performed as indicated in figure legend 1. Scale bar 125 μm. The blots images were cropped prior hybridization and original blots are included in Supplementary Information.